Determination of chlorogenic acid, baicalin and forsythin in shuanghuanglian preparations by hplc-dad

• LI YANG ^[2] ; QINGHUA YAN ^[1] ; HAIZHEN MO ^[3] ; XIAOMIN LI ^[4] ; QING WANG ^[2]
  1. [1] Xinxiang Medical University

     Xinxiang Medical University

     China

     
  2. [2] Henan Institute of Science and Technology Department of Experimental Center
  3. [3] Henan Institute of Science and Technology School of Food Science
  4. [4] Henan Institute of Science and Technology School of Technique and Electricity

  Mostrar afiliaciones +
• Localización: Journal of the Chilean Chemical Society (Boletín de la Sociedad Chilena de Química), ISSN-e 0717-6309, ISSN 0366-1644, Vol. 57, Nº. 4, 2012, págs. 1361-1363
• Idioma: inglés
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• Resumen

  • High performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for the simultaneous determination of three bioactive components - chlorogenic acid, baicalin and forsythin - in traditional Chinese medicinal Shuanghuanglian preparations. The analytes were extracted successfully using 80% aqueous methanol for 15 min in an ultrasonic bath. The optical chromatographic separation conditions were performed on a Finnigan Surveyor HPLC system with an Agilent Zorbax ODS C18 (4.6x150 mm, particle size 5 ìçé ) at 30 ºC using a gradient elution program with a mixture of acetonitrile and 0.2% phosphoric acid (v/v) as mobile phase within 25 min. Method validation results were obtained through the generation of calibration curves with the mixed standard solutions and a standard addition method, regression equations revealed linear relationships (correlation coefficients 0.9965-0.9985) between the peak area and concentration of the three analytes. The average recoveries were 90-106% (n= 6) with standard deviation below 3.37%. The developed HPLC assay was specific and could be readily utilized for comprehensive evaluation of Shuanghuanglian preparations.