Presencia de metalo-ß-lactamasas en Pseudomonas aeruginosa resistente a imipenem

• Alfonso Pérez I ^[1] ; Patricia García C ^[1] ; Helena Poggi M ^[1] ; Stephanie Braun J ^[3] ; Claudia Castillo V ^[1] ; Juan Carlos Román ^[1] ; Marcela Lagos ^[1] ; Eliana Romeo 0 ^[1] ; Lorena Porte T ^[3] ; Jaime Labarca L ^[1] ; Gerardo González R ^[2]
  1. [1] Pontificia Universidad Católica de Chile

     Pontificia Universidad Católica de Chile

     Santiago, Chile

     
  2. [2] Universidad de Concepción

     Universidad de Concepción

     Comuna de Concepción, Chile

     
  3. [3] Hospital Militar Laboratorio de Microbiología

  Mostrar afiliaciones +
• Localización: Revista Médica de Chile, ISSN-e 0034-9887, Vol. 136, Nº. 4, 2008, págs. 423-432
• Idioma: español
• Títulos paralelos:
  • Presence of metal lo ß-lactamases in imipenem-resistant Pseudomonas aeruginosa
• Enlaces
  • Texto completo
• Resumen

  • Background: Metallo-ß-lactamases (MBL) confer high resistance to carbapenems in Pseudomonas aeruginosa (Psae). They are encoded in mobile elements of different genes (VIM, IMP, SMP, GIM), along with other resistance genes. Aim: To detect the presence of MBL in imipenem resistant Psae strains. Material and methods: Fifty-nine imipenem resistant Psae strains isolated from January 2004 to August 2005 in a University Clinical Hospital, were included. The presence of MBL was studied by Etest (phenotypic) and genotypic polymerase chain reaction (PCR) methods. To rule out a nosocomial outbreak, MBL positive strains, were studied by pulse field gel electrophoresis. Results: The presente of MBL was detected in eleven strains. AH were type VIM and were not clonally related. There was no concordance between phenotypic and genotypic MBL detecting methods. AH the strains were also multiresistant. Conclusions: The presence of MBL was detected in 19% of imipenem resistant Psae strains