Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988

• Raquel Diniz Rufino ^[1] ; Juliana Moura de Luna ^[1] ; Galba Maria de Campos Takaki ^[1] ; Leonie Asfora Sarubbo ^[1]
  1. [1] Universidade Católica de Pernambuco

     Universidade Católica de Pernambuco

     Brasil

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 17, Nº. 1, 2014, págs. 6-6
• Idioma: inglés
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• Resumen

  • Background: Biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have been established. Results: The yeast was grown for 72 h to determine the kinetics of growth and production. The surface tension of the cell-free broth was reduced from 55 to 25 mN/m. The yield of biosurfactant was 8.0 g/l with a CMC of 0.03%. The biosurfactant was characterized as an anionic lipopeptide composed of 50% protein, 20% lipids, and 8% of carbohydrates. Conclusions: The isolated biosurfactant showed no toxicity against different vegetable seeds: Brassica oleracea, Solanum gilo and Lactuca sativa L. and the micro-crustacean Artemia salina. The properties of the biosurfactant produced suggest its potential application in industries that require the use of effective compounds at low cost.