Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation

• Fang Ding ^[1] ; Xin Yuan ^[1] ; Qingqing Li ^[1] ; Wenqiang Sun ^[1] ; Chao Gan ^[1] ; Hua He ^[1] ; Chenling Song ^[1] ; Jiwen Wang ^[1]
  1. [1] Sichuan Agricultural University

     Sichuan Agricultural University

     China

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 17, Nº. 6, 2014, págs. 251-261
• Idioma: inglés
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• Resumen

  • Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation. Results We have isolated a 7654 bp fragment from Peking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05). However, 1000 µM treatment of oleic acid exhibited lipotoxic effect on cell viability (P < 0.05). In addition, during the first 24 h of duck adipocyte differentiation FAS was induced; however, after 24 h its expression level declined (P < 0.05). Conclusion We have successfully cloned and characterized Peking duck FAS. FAS was induced during adipocyte differentiation and by oleic acid treatment. These findings suggest that Peking duck FAS plays a similar role to mammalian FAS during adipocyte differentiation.