A rapid and cheap protocol for preparation of PCR templates in peanut

• Chuan Tang Wang ^[1] ; Xiu Zhen Wang ^[2] ; Yue Yi Tang ^[2] ; Jian Cheng Zhang ^[2] ; Shan Lin Yu ^[2] ; Jian Zhi Xu ^[2] ; Zhen Min Bao ^[1]
  1. [1] Ocean University of China

     Ocean University of China

     China

     
  2. [2] Shandong Peanut Research Institute
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 12, Nº. 2, 2009, págs. 9-10
• Idioma: inglés
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• Resumen

  • This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.