Measuring b-Galactosidase activity at pH 6 with a differential pH sensor

• Cristian Acevedo ^[3] ; Matthias H Stach ^[1] ; Anette Amtmann ^[1] ; Manuel E Young ^[3] ; Juan G Reyes ^[2] ; Holger Huebner ^[1] ; Rainer Buchholz ^[1]
  1. [1] University of Erlangen-Nuremberg

     University of Erlangen-Nuremberg

     Kreisfreie Stadt Erlangen, Alemania

     
  2. [2] Pontificia Universidad Católica de Valparaíso

     Pontificia Universidad Católica de Valparaíso

     Valparaíso, Chile

     
  3. [3] Universidad Técnica Federico Santa María Biotechnology Center

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• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 12, Nº. 2, 2009, págs. 12-13
• Idioma: inglés
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• Resumen

  • The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.