Universal protocol for generating 100bp size standard for endless usage

• Chandrasekhar Natarajan ^[1] ; Neetha N Vijayan ^[1] ; Linda Koshy Vaidyan ^[1] ; Anila Mathew ^[1] ; Lekshmy Srinivas ^[1] ; Moinak Banerjee ^[1]
  1. [1] Rajiv Gandhi Centre for Biotechnology Human Molecular Genetics Laboratory
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 11, Nº. 2, 2008, págs. 126-129
• Idioma: inglés
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• Resumen

  • Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.