Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot

Yu-Jin Jung ^[1] ; Chang-Sun Choi ^[1] ; Jin-Heui Park ^[1] ; Hee-Wan Kang ^[1] ; Je-Eul Choi ^[2] ; Ill-Sup Nou ^[3] ; Soon Youl Lee ^[4] ; Kwon-Kyoo Kang ^[1]
1. [1] National University
  
  National University
  
  Estados Unidos
2. [2] Chungnam National University
  
  Chungnam National University
  
  Corea del Sur
3. [3] Sunchon National University
  
  Sunchon National University
  
  Corea del Sur
4. [4] Hankyong National University Genetic Informatics Center, GRRC School of Applied Life Sciences
Mostrar afiliaciones +
Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 11, Nº. 1, 2008, págs. 71-79
Idioma: inglés
Enlaces
- Texto completo
Resumen
- Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1 (the gene encoding fruit bromelain) into a plant expression vector that was then used to transform Brassica rapa and overexpress BAA1 under the control of the cauliflower mosaic virus (CaMV) 35S promoter. We demonstrate that constitutive overexpression of BAA1 in B. rapa confers enhanced resistance to the soft rot pathogen Pectobacterium carotovorum ssp. carotovorum. These results suggest that it could be utilized for protecting plants from attack by bacterial pathogens