Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

• Gregorio Godoy-Hernández ^[1] ; Elidé Avilés-Berzunza ^[1] ; Mildred Carrillo-Pech ^[1] ; Felipe Vázquez-Flota ^[1]
  1. [1] Centro de Investigación Científica de Yucatán Unidad de Bioquímica y Biología Molecular de Plantas
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 11, Nº. 1, 2008, págs. 134-139
• Idioma: inglés
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• Resumen

  • Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation