Development of transformation vectors for the production of potentially high oleate transgenic oil palm

• Mat Yunus Abdul Masani ^[1] ; Ghulam Kadir Ahmad Parveez ^[1]
  1. [1] Malaysian Palm Oil Board Biological Research Division Advanced Biotechnology and Breeding Centre
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 11, Nº. 3, 2008, págs. 23-31
• Idioma: inglés
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• Resumen

  • The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense Δ9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium-based transformation protocols.