Biocatalytic oxidative kinetic resolution of (±)-4-(chlorophenyl)phenylmethanol by Nocardia corallina B-276

• Mario A. Ramírez ^[1] ; Herminia I. Pérez ^[1] ; Norberto Manjarrez ^[1] ; Aida Solís ^[1] ; Héctor Luna ^[1] ; Julia Cassani ^[1]
  1. [1] Universidad Autónoma Metropolitana

     Universidad Autónoma Metropolitana

     México

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 11, Nº. 4, 2008, págs. 2-3
• Idioma: inglés
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• Resumen

  • Chiral diarylmethanols are versatile building blocks for the preparation of biologically active substances, but they are difficult to obtain in enantiopure form. We used Nocardia corallina B-276 for the oxidative kinetic resolution of (±)-4-(chlorophenyl)phenylmethanol, 1. Two experimental methods were used: 1) Suspension of cells in a phosphate buffer solution and 2) Cells in the culture media, in a 3-L bioreactor. After 36 hrs using the first method, the ketone/alcohol ratio was 56/44 and the unoxidized alcohol had an enantiomeric ratio of 93/7, predominating the R-alcohol.