Ethanol mediated enhancement in bacterial transformation

• Arun Dev Sharma ^[1] ; Jaspreet Singh ^[1] ; Prabhjot Kaur Gill ^[2]
  1. [1] Lyallpur Khalsa College Department of Biotechnology
  2. [2] Lovely Institute of Higher Studies
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 10, Nº. 1, 2007, págs. 166-168
• Idioma: inglés
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• Resumen

  • In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.