High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

• Salina Mat Radzi ^[1] ; Mahiran Basri ^[1] ; Abu Bakar Salleh ^[1] ; Arbakariya Ariff ^[1] ; Rosfarizan Mohammad ^[1] ; Mohd. Basyaruddin Abdul Rahman ^[1] ; Raja Noor Zaliha Raja Abdul Rahman ^[1]
  1. [1] Universiti Putra Malaysia

     Universiti Putra Malaysia

     Malasia

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 8, Nº. 3, 2005, págs. 291-298
• Idioma: inglés
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• Resumen

  • High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced