P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis

• M Cecilia Johnson P ^[2] ; Claudio Pinto O ^[2] ; Alessandra Alves L ^[1] ; Alberto Palomino A ^[1] ; Ariel Fuentes G ^[1] ; M Angélica Boric S ^[2] ; Margarita Vega B ^[2]
  1. [1] Universidad de Santiago de Chile

     Universidad de Santiago de Chile

     Santiago, Chile

     
  2. [2] Universidad de Chile Facultad de Medicina Hospital Clínico San Borja Arriarán
• Localización: Revista Médica de Chile, ISSN-e 0034-9887, Vol. 132, Nº. 12, 2004, págs. 1475-1482
• Idioma: español
• Títulos paralelos:
  • P450Arom and estrogenic microenvironment of eutopic endometria in endometriosis
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• Resumen

  • Background: Endometriosis, a common gynecologic disorder characterized by endometrial glands and stroma outside the uterus, is diagnosed by direct visualization of peritoneal and ovarian implants during laparoscopy. Aim: To study the estrogenic microenvironment in eutopic endometria of women with and without endometriosis. Patients and methods: Eutopic endometria, obtained during laparoscopy from 23 women with endometriosis and 20 fertile cyclic women undergoing tubal sterilization, was studied. P450Arom mRNA expression (RT-PCR) was measured. Also, P450Arom activity was assessed measuring testosterone conversion to estradiol and the concentration of this last hormone in cultured endometrial explants. Results: Age and body mass index was similar in both groups studied. Seventy nine percent of endometria from women with endometriosis and in 29.4% from control group expressed P450Arom mRNA (p <0.01). The intensity of the band was higher in secretory endometria from women with endometriosis when compared to controls (p <0.01), but it was similar during the proliferative phase. Estradiol secretion to the culture media by proliferative endometria explants from women with endometriosis was 3-fold higher than secretory endometria (p <0.01) and endometria from control women in both phases. P450Arom activity, in the presence of testosterone, was 7-fold higher in endometrial cultures from women with endometriosis, when compare with the basal culture (p <0.01). However, in endometrial explant cultures from control women, this activity was not statistical different. Conclusions: These results indicate that in women with endometriosis, the microenvironment in the endometria is estrogenic as a consequence of an increased expression and activity of the P450 Arom (Rev Méd Chile 2004; 132: 1475-82).