Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda

• Mauricio Farfán U ; Oscar Flores G ; Niurka Navarro G ; Valeria Prado J ; Guido Mora L ; Cecilia Toro U ^[1]
  1. [1] Universidad de Chile

     Universidad de Chile

     Santiago, Chile

     
• Localización: Revista Médica de Chile, ISSN-e 0034-9887, Vol. 130, Nº. 3, 2002, págs. 275-280
• Idioma: español
• Títulos paralelos:
  • Molecular characterization of resistance mechanisms to chloramphenicol in Shigella flexneri strains isolated from Chilean children with acute diarrhea
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• Resumen

  • Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All CmR strains had a minimal inhibitory concentration over 64 µg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene (Rev Méd Chile 2002; 130: 275-80)