Efecto de la vitamina E(DL alfa-tocoferol) sobre el daño cromosómico en linfocitos de pacientes con ataxia telangiectasia

• Katherine Marcelain C ^[4] ; Carmen Luz Navarrete S ^[5] ; Mireya Bravo L ^[1] ; Manuel Santos A ^[2] ; Cecilia Be R ^[3] ; Juana Pincheira V ^[4]
  1. [1] Universidad de Chile

     Universidad de Chile

     Santiago, Chile

     
  2. [2] Pontificia Universidad Católica de Chile

     Pontificia Universidad Católica de Chile

     Santiago, Chile

     
  3. [3] Clínica Las Condes

     Clínica Las Condes

     Santiago, Chile

     
  4. [4] ICBM Programa de Genética Humana
  5. [5] Hospital Roberto del Río Unidad de Inmunorreumatología Servicio de Pediatría

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• Localización: Revista Médica de Chile, ISSN-e 0034-9887, Vol. 130, Nº. 9, 2002, págs. 957-963
• Idioma: español
• Títulos paralelos:
  • Effect of vitamin E (DL alpha-tocopherol) on chromosomal damage in lymphocytes from patients with ataxia telangiectasia
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• Resumen

  • Background: In ataxia telangiectasia (A-T), the lack of a functional ATM kinase is associated with disturbances in the processing of DNA damage and a chronic oxidative stress. These disturbances may be responsible for an increment of chromosomal damage in A-T cells. Aim: To study the in vitro effect of vitamin E (DL-alpha-tocopherol) on the frequency of chromosomal damage of lymphocytes from patients with A-T. Patients and methods: Seven patients with A-T and age-sex matched controls were studied. Chromosomal damage in mitosis was evaluated in lymphocytes cultures both under basal conditions and when G2 repair was prevented by 5 mM caffeine. Results: In cells from patients with A-T, vitamin E induced a 57.1 and 47.9% decrease in chromosomal damage under basal and inhibited G2 repair conditions, respectively. However, there was a non significant improvement in their repair activity. Vitamin E effects on chromosomal damage was not significant in control subjects. Conclusions: Vitamin E reduces chromosomal damage in lymphocytes from patients with ataxia telangiectasia (Rev Med Chile 2002; 130: 957-63).