Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA

• Rubén Mercado P ^[1] ; María Isabel Jercic L ^[4] ; Patricio Torres H ^[2] ; Sergio Alcayaga U ^[5] ; Fabiana Martins de Paula ^[6] ; Julia María Costa-Cruz ^[3] ; Marlene T Ueta ^[6]
  1. [1] Universidad de Chile

     Universidad de Chile

     Santiago, Chile

     
  2. [2] Universidad Austral de Chile

     Universidad Austral de Chile

     Valdivia, Chile

     
  3. [3] Universidade Federal de Uberlândia

     Universidade Federal de Uberlândia

     Brasil

     
  4. [4] Instituto de Salud Pública de Chile Laboratorio de Referencia de Parasitología
  5. [5] Servicio de Salud Metropolitano Sur Unidad de Epidemiología
  6. [6] Universidad Estadual de Campinas Instituto de Biología Departamento de Parasitología

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• Localización: Revista Médica de Chile, ISSN-e 0034-9887, Vol. 130, Nº. 12, 2002, págs. 1358-1364
• Idioma: español
• Títulos paralelos:
  • Immunodiagnosis of Strongyloides stercoralis infections in Chile using an Elisa test
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• Resumen

  • Background: Strongyloides stercoralis is a world wide distributed small intestinal nematode parasite. In immunocompetent individuals S stercoralis can produce asymptomatic infections or a moderate clinical picture of diarrhea, some cases become chronic. In immunocompromised patients, a disseminated disease may appear, sometimes fatal. In Chile, there is little epidemiological information about S stercoralis infections and appropriate diagnostic techniques are usually not used. Aim: To evaluate the yield of an ELISA test for the diagnosis of strongyloidiasis in Chilean patients. Material and methods: Ten serum samples from patients with S stercoralis infections confirmed by a positive stool examination, 66 samples from individuals with other infections by tissue helminthes (24 toxocariasis, 15 trichinellosis, 11 hydatidosis, 12 fascioliasis and 4 cysticercosis), 13 samples from subjects with autoimmune diseases and 49 samples from apparently healthy individuals with a normal eosinophil count, were studied. ELISA antigen was prepared using a filariform larval extract obtained from a murine species of Strongyloides, maintained in laboratory animals. Results: Using 0.33 optical density units as a cut off value, 9 of 10 sera of S stercoralis infected individuals, had a positive ELISA test. No cross reactions were observed with sera of patients with other helminthic infections, autoimmune diseases or in healthy individuals. Thus, specificity, positive and negative predictive values were 100%. Conclusions: The results obtained are similar with those found by other investigators. ELISA test for strongyloidiasis is a useful tool for the diagnosis of clinical cases and for seroepidemiological studies of this nematode infection in Chile (Rev Méd Chile 2002; 130: 1358-64)