A simple and effective pressure culture system modified from a transwell cell culture system

• Xuerong Sun ^[3] ; Xinguang Liu ^[3] ; Yuehong Zhang ^[1] ; Xielan Kuang ^[1] ; Bo Lv ^[2] ; Jian Ge ^[1]
  1. [1] Sun Yat-sen University

     Sun Yat-sen University

     China

     
  2. [2] Guangdong General Hospital

     Guangdong General Hospital

     China

     
  3. [3] Guangdong Medical College Key Laboratory for Medical Molecular Diagnostics of Guangdong Province Institute of Aging Research

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• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 46, Nº. 1, 2013, págs. 47-52
• Idioma: inglés
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• Resumen

  • Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.