Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain

Qiangcheng Zeng; Yong Guo; Yongming Liu; Ruixin Li; Xinchang Zhang; Lu Liu; Yang Wang; Xizheng Zhang; Xianqiong Zou

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Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain

Qiangcheng Zeng ^[3] ; Yong Guo ^[1] ; Yongming Liu ^[1] ; Ruixin Li ^[2] ; Xinchang Zhang ^[2] ; Lu Liu ^[4] ; Yang Wang ^[1] ; Xizheng Zhang ^[2] ; Xianqiong Zou ^[1]
1. [1] Guilin Medical University
  
  Guilin Medical University
  
  China
2. [2] Academy of Military Medical Sciences
  
  Academy of Military Medical Sciences
  
  China
3. [3] Dezhou University Institute of Biophysics Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics
4. [4] Logistics College of Chinese People's Armed Police Forces Chemistry Department
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Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Nº. 48, 2015
Idioma: inglés
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Resumen
- BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µε) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain.