Antioxidant effect of 5-amino salicylic acid on copper-mediated LDL oxidation

• CAMILO LÓPEZ-ALARCÓN ^[1] ; HERNÁN SPEISKY ^[2] ; EDUARDO LISSI ^[3]
  1. [1] Pontificia Universidad Católica de Chile

     Pontificia Universidad Católica de Chile

     Santiago, Chile

     
  2. [2] Universidad de Chile

     Universidad de Chile

     Santiago, Chile

     
  3. [3] Universidad de Santiago de Chile

     Universidad de Santiago de Chile

     Santiago, Chile

     

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• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 40, Nº. 2, 2007, págs. 155-162
• Idioma: inglés
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• Resumen

  • The antioxidant effect of 5-Aminosalicylic acid (5-ASA) on copper-mediated LDL oxidation was followed either by the emitted chemiluminiscence (CL) or by UV-vis spectroscopy. 5-ASA addition extends the lag phase in a concentration-dependent manner without changes in the rate of the process in the autoaccelerated phase. The antioxidant behavior of 5-ASA was very similar to that of Trolox, a very efficient water soluble antioxidant. The copper-binding capacity of 5-ASA was evaluated by UV-visible spectroscopy. The addition of copper to a 5-ASA solution increases the absorbance at 332 nm and generates a new band at 298 nm. These changes in the UV-vis spectra indicate formation of a complex between 5-ASA and copper. However, LDL protection by 5-ASA is unrelated to its copper chelating capacity