A novel seed baiting technique for the epiphytic orchid rhynchostele cer vantesii, a means to acquire mycorrhizal fungi from protocorms

• Jesus Bernardo Cruz-Higareda ^[1] ; Barbara Susana Luna-Rosales ^[1] ; Amadeo Barba- Alvarez ^[1]
  1. [1] Universidad Nacional Autónoma de México

     Universidad Nacional Autónoma de México

     México

     
• Localización: Lankesteriana, ISSN-e 1409-3871, Vol. 15, Nº. 1, 2015, págs. 67-76
• Idioma: inglés
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• Resumen

  • We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.