Resumen de Development of Twenty Novel Polymorphic Microsatellite Markers and Their Application in Population Genetic Studies of Konosirus punctatus
Zengliang Miao, Xun Jin, Shiyi Chen, Kun Zhang, Jiasheng Li, Ying Peng, Wenhua Huang, Xudong Liang, Haodi Shen, Yifan Liu, Bingjian Liu
Konosirus punctatus is an important fishery resource which is distributed from the Indian Ocean to the northwest Pacific. To study the genetic diversity of K. punctatus, we designed forty microsatellite markers based on Illumina and PacBio sequencing data. Twenty of them (50%) were successfully amplified and exhibited polymorphism which were selected for population genetic analyses in three K. punctatus populations from China (Xiamen, Zhoushan, and Laizhou). The number of alleles per locus ranged from 8 to 15, and the values of expected heterozygosity (He) and observed heterozygosity (Ho) were 0.454 ~ 0.918 (mean = 0.775) and 0.125 ~ 0.958 (mean = 0.706), respectively. The FST showed that the three geographic populations were moderately differentiated, while Structure and PCoA analysis showed that there was no significant differentiation between pairwise populations. These microsatellite markers will provide a basis for the study of the population genetics of K. punctatus.
