Studies on parasitemia courses and mortality in mice infected with genetically distant Trypanosoma cruzi clonets

• ANNE WALLACE ^[1] ; SILVIA ORTÍZ ^[2] ; GITTITH SÁNCHEZ ^[2] ; REBECA VILLAGRA ^[1] ; MABEL MUGA ^[1] ; ALDO SOLARI ^[1]
  1. [1] Universidad de Chile

     Universidad de Chile

     Santiago, Chile

     
  2. [2] Program of Cellular and Molecular Biology
• Localización: Biological Research, ISSN-e 0717-6287, ISSN 0716-9760, Vol. 34, Nº. 2, 2001, págs. 83-90
• Idioma: inglés
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• Resumen

  • The biological characterization of bloodstream forms of eleven Trypanosoma cruzi cloned stocks, corresponding to two genetically similar clonets and one distant clonet (39), according to multilocus enzyme electrophoresis analysis, showed dissimilar parasitemia in an experimental isogenic mouse model. While clonet 39 stocks gave low parasitemias, clonets 19 or 20 stocks gave high parasitemias, independently of the inocula (10² and 10(4) bloodstream forms) used. High parasitemia did not always associate with greater mortality. Statistical studies on mortality using a low inocula showed significantly higher mortality with clonet 39 stocks when compared to clonets 19 or 20 stocks. Finally, in order to confirm the identity of each stock studied, typing by molecular karyotype was performed before inoculating mice.