Resumen de Rutin increases neural crest stem cell survival against damage caused by aflatoxin B1

Jader Nones, Janaína Nones, Andréa G. Trentin

• English

  The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potential. The derivatives of the NC at the trunk level include neurons and glial cells of the peripheral nervous system, melanocytes, smooth muscle cells and some endocrine cells. The present work investigated, for the first time, the influence of aflatoxin B1 (AFB1) and the flavonoid rutin on the survival and proliferation of NC and NC-derived melanocytes. Quail NC cell cultures were treated with AFB1 (30 ?M) and/or rutin (20 ?M) for 6 days. Cell viability was assessed by MTT and trypan blue analyses and cell proliferation by BrdU staining. Melanocytes were identified by immunocytochemistry against the melanocyte-specific cellular marker MelEM. The AFB1 treatment decreased both NC cell viability and proliferation. The total number of MelEM-positive cells was also reduced after this treatment, an effect partially prevented by the addition of rutin. On the other hand, rutin added alone did not influence the NC cell population. Our results demonstrated that rutin increases the survival of the NC after damage caused by AFB1. However, additional studies are needed to better understand the mechanisms involved in AFB1 and rutin interactions.

• português

  A crista neural (CN) corresponde a um conjunto de células progenitoras multi e oligopotentes dotadas com potenciais neural e mesenquimal. Os derivados da CN incluem neurônios e células gliais do sistema nervoso periférico, melanócitos, células da musculatura lisa e algumas células endócrinas. No presente trabalho, investigamos pela primeira vez a influência da aflatoxina B1 (AFB1) e do flavonoide rutina na sobrevivência e proliferação da CN e de melanócitos derivados deste tipo celular. Para tal, culturas de células da CN de codornas foram tratadas com AFB1 (30 ?M) e/ou rutina (20 ?M) durante seis dias. A viabilidade celular foi avaliada por análises de MTT e azul de tripan e a proliferação celular através de marcação com BrdU. Melanócitos foram identificados com uso do marcador celular específico MelEM. O tratamento com a AFB1 diminuiu a viabilidade e proliferação das células da CN. O número total de células MelEM-positivas foi também reduzido após este tratamento, efeito parcialmente revertido através da adição de rutina. No entanto, uma melhor compreensão referente aos mecanismos envolvidos nas interações entre AFB1 e rutina precisarão ser realizados.