Strategies for the production of cationic ?-helical antimicrobial peptides in plant biofactories
- Autores: Núria Company Casadevall
- Directores de la Tesis: Anna Nadal i Matamala (dir. tes.), Maria Pla i de Solà-Morales (dir. tes.)
- Lectura: En la Universitat de Girona ( España ) en 2014
- Idioma: inglés
- Tribunal Calificador de la Tesis: Pere Puigdomènech i Rosell (presid.), Dolors Parés i Oliva (secret.), Anna Coll Rius (voc.)
- Materias:
- Enlaces
- Tesis en acceso abierto en: TDX
- Resumen
Antimicrobial Peptides have strong interest as a novel class of antimicrobial agents in the phytosanitary field. This Thesis focuses on the development of strategies to produce of BP100 derivatives (BP100ders) in genetically modified (GM) rice plant biofactories. The production of certain BP100ders using a strong constitutive promoter was toxic to the host plant and, impairing its viability. That phytotoxicity to the host was directly linked to the haemolytic activity of BP100ders. Transgenic plants were obtained that produced these peptides with yields up to 0.5% total soluble protein and retaining their antimicrobial activity. Through fusion to the DsRed fluorescent tag allowed tracing recombinant BP100ders in plants observing the accumulation in large vesicles derived from the endoplasmic reticulum. Through a strategy based on inducible promoters, particularly heat-shock, we showed that recombinant phytotoxic peptides can be produced in GM plants by using promoters that have minimal activities during transformation and regeneration of GM plants
