Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis

• Autores: Alessandro Pinto
• Directores de la Tesis: Ciara K. O'Sullivan (dir. tes.)
• Lectura: En la Universitat Rovira i Virgili ( España ) en 2012
• Idioma: inglés
• Tribunal Calificador de la Tesis: Ioanis Katakis (presid.), Alexander Heckel (secret.), Sara Tombelli (voc.)
• Materias:
  • Química
    • Química analítica
  • Ciencias de la vida
    • Biología molecular
• Enlaces
  • Tesis en acceso abierto en: TDX
• Resumen

  • The thesis aimed to develop and characterize a novel detection approach, which we termed aptaPCR exploiting nucleic acid aptamers as combined recognition and reporter biocomponents for the ultrasensitive detection of analytes. Nucleic acid aptamers are synthetic ligands selected from vast combinatorial libraries through a process referred to as SELEX – Systematic Evolution of Ligand By Exponential Enrichment. As compared to other natural and synthetic receptor, aptamers possess unique chemical and biochemical characteristics, such as: a well known chemistry, remarkable stability, an ability to be selected against virtually any target even in non-physiological conditions