F-actin cytoskeleton functional architecture and dynamics in secretion in neuroendocrine model

• Autores: Yolanda Giménez Molina
• Directores de la Tesis: Jose Heliodoro Villanueva Roig (dir. tes.), Luis Miguel Gutiérrez Pérez (codir. tes.)
• Lectura: En la Universidad Miguel Hernández de Elche ( España ) en 2018
• Idioma: español
• Tribunal Calificador de la Tesis: Juan Antonio Reig Maciá (presid.), Sandra Jurado Sánchez (secret.), Eva Alés González de la Higuera (voc.), Amparo Gil Gómez (voc.), Bazbek Davletov (voc.)
• Programa de doctorado: Programa de Doctorado en Biología Molecular y Celular por la Universidad Miguel Hernández de Elche
• Materias:
  • Ciencias de la vida
    • Biología celular
• Enlaces
  • Tesis en acceso abierto en: RediUMH (pdf)
• Resumen

  • The PhD thesis submitted by the PhD student Yolanda Giménez Molina is focused on F-actin cytoskeleton study in terms of structure and dynamics. The objective is expanding the knowledge about the F-actin distribution and dynamics role in neurosecretion process.

    Firstly, this thesis tries to improve the F-actin microfilaments cytoarchitecture description including last scientific findings about F-actin cytoplasmic organization in native chromaffin cells from bovine adrenal medulla, comparing the F-actin architecture from isolated chromaffin cells and showing the influence on vesicles and mitochondria distribution inside cell by F-actin configuration. Secondly, the thesis describes F-actin local dynamics by stimulation, not only in terms of fibbers displacements but also for cytoskeletal network´s cavities remodeling under active zones. Moreover, this work explains and reports new transport systems for vesicles and mitochondria linked to F-actin fibbers, especially to F-actin dynamics and fibbers polymerization potential.

    In order to reach all these objectives, chromaffin cells have been selected as a neuroendocrine cellular model accepted for secretion studies and a multidisciplinary set of techniques have been used, combining: biochemistry and molecular biology tools, biophysical analysis with a high temporal resolution of secretion and bioimaging by dynamic confocal microscopy.