NMR studies of the interactions of glycan antigens with monoclonal antibodies
- Autores: Ilaria Calloni
- Directores de la Tesis: Niels-Christian Reichardt (dir. tes.), María Nuria Sotomayor Anduiza (tut. tes.), Jesús Jiménez Barbero (dir. tes.)
- Lectura: En la Universidad del País Vasco - Euskal Herriko Unibertsitatea ( España ) en 2020
- Idioma: inglés
- Tribunal Calificador de la Tesis: Juan Bosch Cartes (presid.), Alma Rosa López Álvarez (secret.), Maria Esther Lete Exposito (voc.), Francisco Corzana López (voc.), Miquel Angel Pericàs (voc.)
- Programa de doctorado: Programa de Doctorado en Química Sintética e Industrial por la Universidad del País Vasco/Euskal Herriko Unibertsitatea y la Universidad Pública de Navarra
- Materias:
- Enlaces
- Tesis en acceso abierto en: ADDI
- Resumen
Carbohydrates are one of the most variable and complex molecules of biological systems. They are usually located at the cell membrane, mainly as glycoconjugates, glycoproteins or glycolipids and play an important role in the specific molecular recognitions. Within glycans, polysaccharide capsules (CPS) are universal structures found on the cell surface of a broad range of bacterial species and often constitute the outermost layer of the cell; as such, they mediate the direct interactions between the bacterium and its immediate environment. Since structure and function are intrinsically correlated, the three-dimensional shape, dynamics and presentation of these molecules play a role in the recognition processes.In this work, we have employed a variety of Nuclear Magnetic Resonance spectroscopy (NMR) techniques, assisted by different computational approaches, in particular Molecular Dynamics (MD) simulation and ab initio methods, for obtaining atomic-scale information on the conformation, dynamics and interactions, in solution, of different types of capsular polysaccharides present in Neisseria meningitis and Streptococcus B.
