Veiled chameleon (Chamaeleo calyptratus) and Panther chameleon (Furcifer pardalis) are the most popular chameleons over the world, and consequently, two of the most frequently species attended in veterinary practice.
The most commonly used imaging techniques in diagnostic protocols in herpetology include radiography, ultrasonography (US), computed tomography (CT) and magnetic resonance imaging (MRI). However, few studies about imaging description in reptiles have been published.
The objectives of this prospective anatomic study were to develop imaging techniques for these species and to describe the normal anatomy of the coelomic organs using radiography, US and CT scan.
Seventeen healthy veiled chameleons (7 males and 10 females) and fifteen healthy panther chameleons (13 males and 2 females) were included in the study. Animals were considered to be healthy on the basis of the results of a complete physical examination and coprology testing.
The imaging study was performed in sedated chameleons after the administration of alfaxalone (Alfaxan®, Crawley, UK) 4-6 mg/kg IV in the ventral vein of the tail. A right lateral and dorsoventral radiographic views were performed with a mammography cassette plate in all animals. Then, an ultrasound was performed in right lateral recumbency using a linear 15 to 18-MHz transducer. Finally, a CT examination was performed with a 16-slice helical CT scanner in sternal recumbency. Post-mortem study of one chameleon of each species were used to clarify and illustrate coelomic anatomy and to assess imaging findings.
The results of the current study suggest that the different imaging techniques (radiography, ultrasonography and computed tomography) allow the visualization of the liver (including caudal vena cava and hepatic veins), gallbladder, stomach, intestines, gonads, fat bodies and kidneys in healthy veiled and panther chameleons. The urinary bladder was identified using US and CT examination. The spleen, pancreas and adrenal glands were identified in post-mortem studies, but could not be visualized with any imaging technique.
In conclusion, this study provides a guide of the normal imaging anatomic features of the coelomic organs in veiled chameleons and panther chameleons. Findings can be used as a reference for future research studies or for examinations of clinically ill patients.
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