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ISG15 at crossroads of immune synapse, dendritic cells and contact hypersensitivity

  • Autores: Irene Fernández Delgado
  • Directores de la Tesis: Francisco Sánchez Madrid (dir. tes.)
  • Lectura: En la Universidad Autónoma de Madrid ( España ) en 2022
  • Idioma: inglés
  • Número de páginas: 286
  • Tribunal Calificador de la Tesis: Manuel Fresno Escudero (presid.), Susana Guerra García (secret.), José Luís Rodríguez Fernández (voc.), Andrés Alcover Santos (voc.), Salvador Iborra Martín (voc.)
  • Programa de doctorado: Programa de Doctorado en Biociencias Moleculares por la Universidad Autónoma de Madrid
  • Materias:
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  • Resumen
    • Understanding the fate of dendritic cells (DCs) after productive immune synapses (postsynaptic DCs) with T cells during antigen presentation has been largely neglected in favour of deciphering the nuances of T cell activation and memory generation. Here, we describe that postsynaptic DCs switch their transcriptomic signature, correlating with epigenomic changes including DNA accessibility and histone methylation. We focused on the chemokine receptor Ccr7 as a proof-of-concept gene that is increased in postsynaptic DCs. Consistent with our epigenomic observations, postsynaptic DCs migrate more efficiently toward the CCR7 ligand, the chemokine CCL19, in vitro and display enhanced homing to draining lymph nodes (dLN) in vivo.

      Furthermore, these DCs increase the expression of antiviral genes such as Isg15, which confers protection against subsequent viral infections. Henceforth, we assessed the role of ISG15 in DCdeficient for this gene. Although, reduced MHCII surface expression is found after LPS maturation, no significant differences in DCs maturation were observed in response to TLR agonists. Moreover, Isg15deficient DCs secrete reduced amounts of pro-inflammatory cytokines, including IL-1β and IL-12. Once a DC is mature, it will take the path towards a dLN. However, the lack of ISG15 does not affect DCs migration capability towards CCL21 in vitro or dLNs in vivo. Once in the dLN, DCs carrying a specific antigen present it via MHC to T cell lymphocytes which are not affected in terms of T cell activation and proliferation when ISG15 is absent. Thus, ISG15 absence is apparently affecting DC cytokine secretion. However, the specific mechanism is still uncovered. It would be of great interest to decipher whether this effect is due to ISG15 conjugation to proteins or its role as a free soluble molecule.

      Finally, over the years ISG15 has been on the spotlight of studies on pathogen infection or cancer development. However, recent studies suggest a role in inflammatory and other immune diseases. Isg15 is up-regulated in human psoriatic skin and mutations in this gene display dermatological alterations. Here, we observed that the absence of ISG15 leads to a reduced inflammation and swelling in a murine model of allergic contact dermatitis (ACD). Our data indicate that immune cells derived from bone marrow, and particularly DCs might be the immune cell subset accounting for this effect.

      Hence, our work describes a previously unknown DC population whose transcriptomic, epigenomic profiles, and migratory capacity change in response to their cognate contact with T cells. In addition, we found that the lack of ISG15 in DCs provokes a reduced pro-inflammatory cytokine secretion and reduced inflammation in a model of ACD. This study can open new avenues to potentiate immunotherapies based on DCs or develop new therapeutics to treat certain immune dermatological condition


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