Evaluation of the biophysical performance upon the integration of helper components into cationic niosomes for the treatment of both retinal and brain diseases by gene therapy approach

• Autores: Nuseibah Hasan Flayyeh Al Qtaish
• Directores de la Tesis: Gustavo Puras Ochoa (dir. tes.), José Luis Pedraz Muñoz (dir. tes.)
• Lectura: En la Universidad del País Vasco - Euskal Herriko Unibertsitatea ( España ) en 2023
• Idioma: inglés
• Programa de doctorado: Programa de Doctorado en Investigación y Evaluación de Medicamentos. Aplicación de la Tecnología Farmacéutica al Desarrollo de Terapias Avanzadas por la Universidad del País Vasco/Euskal Herriko Unibertsitatea
• Materias:
  • Ciencias de la vida
    • Biología celular
      • Cultivo celular
    • Genética
      • Ingeniería genética
  • Ciencias agrarias
    • Ciencias veterinarias
      • Genética
• Enlaces
  • Tesis en acceso abierto en: ADDI
• Resumen

  • The integration of novel compounds into formulations may open up new avenues andopportunities for improving the treatment of various diseases using a gene therapytechnique. To this purpose, my thesis aimed to study the impact of incorporation of biomaterialssuch sphingolipids or nanodiamonds as a helper component into a cationic niosome formulationfor gene delivery, the formulations and related complexes were studied in terms of particle size,surface charge, dispersity, and morphology. Furthermore, biological research were conducted inboth in vitro and in vivo experimental trials to evaluate cellular uptake, intracellular traffickingpathways, and transfection efficiency.