Anàlisi informatitzada de semen en èquids

• Autores: Sabrina Gacem
• Directores de la Tesis: Jordi Miró Roig (dir. tes.), Carles Soler Vazquez (codir. tes.)
• Lectura: En la Universitat Autònoma de Barcelona ( España ) en 2021
• Idioma: español
• Tribunal Calificador de la Tesis: Graça Maria Ferreira Dias (presid.), María Montserrat Rivera del Álamo (secret.), Marc Yeste Oliveras (voc.)
• Programa de doctorado: Programa de Doctorado en Medicina y Sanidad Animales por la Universidad Autónoma de Barcelona
• Materias:
  • Ciencias agrarias
    • Ciencias veterinarias
      • Cirugía
• Enlaces
  • Tesis en acceso abierto en: TDX
• Resumen

  • The approach advocated in this thesis is based on the fact that there is a gap of information regarding optimization of breeding soundness examination along with semen analysis protocols in equids as seen in the previous chapter. In donkey, no specific protocols for semen analysis were defined, only for stallion samples where there is a general agreement about the protocol to use. When the reproductive tract is checked, B-mode ultrasonography is used as a non-invasive and easy diagnostic method. On the other hand, pulsed Doppler ultrasonography can be used to study testicular blood perfusion. Pulsed-Doppler indices of testicular artery and ASG sizes showed a positive correlation with semen quality in stallion and different species. Unfortunately, no such studies were done in donkey, which makes up the first objective of the thesis. However, when it’s about semen analysis, CASA technology represents the best objective method used today. It permits to obtain reliable quantitative data, even if it is needed to define protocols assuring the consistency and universal application of the results. However, this standardization has never been done following an integrative point of view, which composes the second general objective. There are three main aspects to consider when optimizing automated semen analyses by CASA-Mot technology, namely the type, depth of the counting chamber, the dilution, and the frame rate of image acquisition. The advances in technology allowed now the acquisition of high capture frequency and at the same time the process of these images in seconds. Also, was defined sperm subpopulations structure at higher frames and SP distribution between stallions’ breeds. The third objective was to collaborate in the use and the improvement of the new Trumorph® technique, for sperm morphology analysis which permits the analysis of alive immobilized sperm.