Resumen de Direccionamiento de nanotubos de carbono multipared a células tumorales HER2 positivas
- español
Los nanotubos de carbono de múltipared oxidados (ox-MWCNTs) poseen propiedades fisicoquímicas y biológicas extrordinarias. Además, su estructura de nanofilamento tubular y su amplia superficie permite que el nanotubo sea capaz de capturar múltiples biomoléculas. Por ello, el control de la biocorona es fundamental en la nanobiotecnología de los ox- MWCNTs. Aquí, hemos estudiado los posibles factores que afectan a esa biocororona. Teniendo en cuenta estas propiedades, utilizamos nanotubos como mediadores en la terapia del cáncer mediante su funcionalización con proteínas recombinantes que hemos diseñado y que se unen a HER2 (un receptor sobre-expresado en la mayoría de las células cancerosas) o VEGFR (expresado en las células formadoras de la neovasulatura). De esa manera podremos dirigir estos nanomateriales a los tumores e interferir en su crecimiento.
- English
Carbon nanotubes (CNTs) intrinsic physicochemical properties, such as elemental composition, resilience, thermal properties, and surface reactivity, make them a favorable tool in nanotechnology and biotechnology research.
CNTs are able to capture biomolecules on their surface; therefore, they have become a sectional tool in targeted drug delivery. A quantitative analysis using TGA revealed that 20-60% of the mass of functionalized CNTs and 43% of the mass of functionalized graphene oxide (GO) corresponded to their protein coating when exposed to biological environments.
This biological coating grants them the capacity to interact with a number of conspicuous cell receptors to penetrate membranes and interfere with cell biomechanics. Consequently, bio-camouflage turns CNTs into a biomimetic structure with unpredictable behavior. Our study reveals that the protein content of biocorona significantly changes under various conditions. This variability depends away others on functionalization temperature, the types of CNTs, or the protein content of the surrounding environment. Therefore, we conclude that the CNT biocorona is unpredictable, so its control is essential to target CNTs to favorable destinations and prevent unwanted protein interactions.
In this regard, we have designed two recombinant fusion proteins containing a sequence that allows efficient functionalization of ox-MWCNTs and GO. These proteins are also fused to a fluorescent protein (GFP), and two peptides; a receptor-binding peptide for HER2, expressed in many types of cancer; and a peptide recognizing VEGFR, expressed in the metastatic neovasculature. Recombinant proteins were produced and purified for ox-MWCNT and GO functionalization. Our study demonstrates the stability of this coating and how it avoids nonspecific interactions with proteins in the biological environment. In conclusion, here we show an efficient way to functionalize CNTs for biomedical applications.
