Structural analysis of macromolecular nanomachines by 3d electron microscopy: Managing flexibility and heterogenety in some define cases
- Autores: Ana Lucía Álvarez Cabrera
- Directores de la Tesis: Carlos Óscar Sánchez Sorzano (dir. tes.), José María Carazo García (tut. tes.)
- Lectura: En la Universidad Autónoma de Madrid ( España ) en 2015
- Idioma: español
- Tribunal Calificador de la Tesis: José López Carrascosa (presid.), Daniel Luque Buzo (secret.), Jasminka Boskovic (voc.), Xiaojiang S. Chen (voc.), Óscar Antonio Llorca Blanco (voc.)
- Materias:
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- Resumen
The term macromolecular nanomachines refers to those complexes that correspond to the functional machinery in the context of the living cell,, from DNA repair or replication, to protein degradation or cell división, to name just a few functions. Normally, they are formed by different types of proteins and may also include nucleic acids. The estructure of macromolecular nanomachines and their structural changes is a very important process with a vital role in functional implications. Their study is many times accomplished by three-dimensional elecron microscopy, since they are too large for Nuclear Magnetic Resonance and too flexible for X-ray Diffraction. In these termes, Electron microscopy has provided important structdural information about protein complexes essential for describing their functional organization. Understanding how function occurs can only be inferred after integrating many different types of data (extracto del informe del doctor Don Carlos Óscar Sorzano Sánchez).
