Unravelling cell-particle interactions for the design of new micro- and nanoengineered systems

• Autores: Tania Patiño Padial
• Directores de la Tesis: María Elena Ibáñez de Sans (dir. tes.), L. Barrios (dir. tes.), C. Nogués i (dir. tes.)
• Lectura: En la Universitat Autònoma de Barcelona ( España ) en 2015
• Idioma: inglés
• Tribunal Calificador de la Tesis: Laura Tusell Padrós (presid.), Arántzazu González Campo (secret.), Raghavendra Palankar (voc.)
• Programa de doctorado: Programa Oficial de Doctorado en Biología Celular
• Materias:
  • Ciencias de la vida
    • Biología celular
• Enlaces
  • Tesis en acceso abierto en:  TDX  DDD 
• Resumen

  • The unique and controllable physico-chemical properties of micro- and nanomaterials have allowed the creation of ground-breaking approaches to overcome some of the major challenges of different branches of technology and science, including modern medicine. The fast advances in micro- and nanotechnology have lead to an increasing demand for understanding the behaviour of micro- and nanomaterials within the physiological environments as well as their interactions at the bio-interface, including the cellular level, for an efficient and safe development. In this scenario, the present Thesis aimed to provide an integrated understanding about microparticle interactions with cells. First, we assessed the impact of cationic lipids and polymer coating of microparticles on their uptake by non-phagocytic (HeLa) cells. We found that non-covalently conjugated PEI at a 0.05 mM concentration offers the best balance between uptake efficiency and cytotoxicity. Second, we observed that surface modified microparticles were differently uptaken by tumoral (SKBR-3) and nontumoral (MCF-10A) human breast epithelial cells, not only in terms of uptake efficiency but also of their endocytic pathways. Third, we demonstrated that polysilicon-chromium-gold multi-material intracellular chips (MMICCs) are suitable for biological applications due to their biocompatibility and capability of developing multiple functions through their bi-functionalization using orthogonal chemistry. Collectively, our results highlight the importance of assessing cell-particle interactions, in terms of cytotoxicity, uptake, intracellular location and cell type effect of newly designed micro- and nanomaterials, not only with respect to the target cells but also the neighbouring cells, in order to ensure their safety and efficiency