Antimicrobial activity in Bacillus spp. from plant environments against plant pathogens. Relationship with cyclic lipopeptide genes and products
- Autores: Isabel María Mora Pons
- Directores de la Tesis: Emilio Montesinos Seguí (dir. tes.), Jordi Cabrefiga (dir. tes.)
- Lectura: En la Universitat de Girona ( España ) en 2013
- Idioma: inglés
- Tribunal Calificador de la Tesis: Antonio de Vicente Moreno (presid.), Anna Bonaterra Carreras (secret.), Blanca Beatriz Landa del Castillo (voc.)
- Materias:
- Enlaces
- Tesis en acceso abierto en: TDX
- Resumen
Bacillus subtilis and related species have strong interest in agriculture because several strains are biocontrol agents of plant diseases, which have been recognized as biosafe. In this work, a selective enrichment procedure based on PCR targeting antimicrobial peptide (AMP) genes has been designed and evaluated to screen field samples and pure cultures of Bacillus spp. The procedure increased the yield of isolation of Bacillus strains compared to the standard procedure. The isolates were characterized according to the presence of six AMP genes, the capacity to produce AMPs, and their antimicrobial activity against fungal and bacterial plant pathogens. The relationships between AMP genes, products and antimicrobial activity have been established. Finally, a collection of 184 Bacillus has been obtained having multiple AMP genes simultaneously, and producing several cyclic lipopeptides, with a wide range of antimicrobial activity. It is expected that such strains would be suitable candidates to develop novel biopesticides for plant disease control
