Resumen de Detection of marine toxins using cell-based assays and Characterization of toxin profiles in ciguatera-related natural samples: microalgae and fish. / (Detección de toxinas marinas mediante ensayos celulares y Caracterización del perfil toxinico en muestras naturales asociadas a la ciguatera: microalgas y pescado

Amandine Caillaud

• The suitability of neuroblastoma cell-based assays (Neuro-2a CBAs) was examined in order to characterize toxin profiles in ciguatera-related natural samples: microlagae (Gambierdiscus spp. and Prorocentrum rhathymum) and ciguatera-suspected fish samples from the Canary Islands. The elimination of the interferences of biological matrices and the specificity of the Neuro-2a CBA for ciguatoxins and maitotoxins were two aspects examined to favor the applicability of the Neuro-2a CBA for marine toxins detection. The results presented in this work have contributed to ciguatera risk assessment in the Canary Islands and support the use of CBAs as an alternative or complementary approach to the mouse bioassay for the determination of marine toxins.