Resumen de An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory
Robert J. Olsen, Julie A. Olson, Greta A. Giles
An experiment using 1H NMR spectroscopy to observe the kinetics of the acylase 1-catalyzed hydrolysis of N-acetyl-dl-methionine has been developed for the organic laboratory. The l-enantiomer of the reactant is hydrolyzed completely in less than 2 h, and 1H NMR spectroscopic data from a single sample can be worked up to generate Michaelis−Menten and Lineweaver−Burke kinetic plots. The experiment introduces organic chemistry students to the use of a 1H NMR spectrometer, serves as an introduction to enzyme kinetics, and illustrates the stereospecificity of enzyme catalysis
