Preparation of Buffers. An Experiment for Quantitative Analysis Laboratory

• P. T. Buckley ^[1]
  1. [1] Hartwick College

     Hartwick College

     City of Oneonta, Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 78, Nº 10, 2001, pág. 1384
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • In our experience, students who have a solid grounding in the theoretical aspects of buffers, buffer preparation, and buffering capacity are often at a loss when required to actually prepare a buffer in a research setting. However, there are very few published laboratory experiments pertaining to buffers. This laboratory experiment for the undergraduate quantitative analysis lab gives students hands-on experience in the preparation of buffers. By preparing a buffer to a randomly chosen pH value and comparing the theoretical pH to the actual pH, students apply their theoretical understanding of the Henderson-Hasselbalch equation, activity coefficients, and the effect of adding acid or base to a buffer. This experiment gives students experience in buffer preparation for research situations and helps them in advanced courses such as biochemistry where a fundamental knowledge of buffer systems is essential.