BACKGROUND: Soy yoghurts containing enhanced bioactive isoflavone aglycones were prepared from combinations of yellow (YS) and black soymilk (BS) (YS:BS = 100:0, 70:30, 50:50 and 0:100, v/v) by fermentation with Streptococcus infantarius 12 and Weisellia sp. 4 at 37 °C for 10 h. Sensory properties of the bioactive soy yoghurts were investigated. Furthermore, other characteristics including the contents of isoflavones, viable cell numbers, pH, colour and DPPH radical scavenging activity were investigated. RESULTS: One hundred percent YS yoghurt showed the highest scores of all sensory attributes while 100% BS yoghurt was the lowest for colour and smoothness. The overall acceptability scores were 5.9 and 5.2 for 100% YS and 100% BS yoghurts, respectively (9-point scale). The L-values of soy yoghurts decreased as the percentage BS increased, indicating darkening of soy yoghurts. The contents of bioactive isoflavone aglycones (daidzein and genistein) in soy yoghurts remarkably increased (>1300 mg kg−1) by fermentation. Viable cell numbers and pH of soy yoghurts ranged from 8.4 × 108 to 9.6 × 108 CFU g−1 and 4.43 to 4.54, respectively. DPPH radical scavenging activity significantly increased as the percentage BS increased (P < 0.05). CONCLUSION: One hundred percent YS yoghurt was better for overall sensory properties while 100% BS yogurt was more bioactive in terms of the isoflavone aglycone contents and DPPH radical scavenging activity compared to other yoghurts. Copyright © 2008 Society of Chemical Industry
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