Resumen de New Procedure To Readily Investigate Lactase Enzymatic Activity Using Fehling’s Reagent
Rocco Leonello, Matteo Savio, Paola Baron Toaldo, Renato Bonomi
Determination of enzymatic activity is a relevant experiment in teaching chemistry and biochemistry to high school and college students. A method for measuring the kinetic parameters of lactose cleavage by the enzyme lactase is described. This enzyme catalyzes the hydrolysis of lactose to galactose and glucose. The kinetic parameters can be determined easily and quickly by titration using Fehling’s test for reducing sugars. In this way, it is possible to determine the rate of the reaction without using any instruments.
