Characterization of Extracellular Polymeric Substances Produced by Pseudomonas fragiUnder Air and Modified Atmosphere Packaging
- Autores: Guang‐Yu Wang, Fang Ma, Hu‐Hu Wang, Xing-Lian Xu, Guang-Hong Zhou
- Localización: Journal of food science, ISSN 0022-1147, Vol. 82, Nº 9, 2017, págs. 2151-2157
- Idioma: inglés
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Resumen
Extracellular polymeric substances (EPS) play an important role in bacterial biochemical properties. The characteristics of EPS from 2 strains of Pseudomonas fragicultured in meat aerobically (control) and in modified atmosphere packaging (MAP) were studied. The amount and components of EPS, the surface properties, and the effect on biofilm formation of several spoilage organisms were evaluated. The results showed that MAP inhibited the growth of the P. fragistrains. Compared with the control, more loose and less bound EPS (containing protein and carbohydrate) were produced by P. fragiin MAP samples. MAP also caused increased cell autoaggregation and surface hydrophobicity. After the removal of the EPS, the surface property changes were strain‐dependent, suggesting that membrane compositions were also changed. In addition, the EPS displayed significant antibiofilm activity on Pseudomonas fluorescensand Serratia liquefaciens. In conclusion, P. fragistrains not only modified the amount, components, and surface properties of EPS but also changed the cell membrane compositions to adapt to MAP stress. Moreover, EPS may play an important role in microbial community competitions. This study evaluated the formation of EPS secreted by P. fragiin meat under modified atmosphere packaging. The results highlight that the storage conditions have an impact on the EPS properties of P. fragi, and EPS may play an important role in P. fragiregarding its better growth under aerobic conditions and survival under anaerobic conditions. This work improved the spoilage theory and suggested that meat preservation strategies could be studied from the perspective of EPS.
