Background and Aims: Grape phylloxera, Daktulosphaira vitifoliae (Fitch), is a significant insect pest of grapevines and can cause mild to severe economic damage to ungrafted European grapevine, Vitis vinifera L., depending on the insect clonal type. Dry heat treatment is currently recommended as a disinfestation method to restrict the spread of phylloxera to uninfested vineyards on viticultural machinery.
Methods and Results: G1 and G4 first instar grape phylloxera clones were subjected to two relative humidity (30 and 100%) and four temperature (30, 35, 40 and 45°C) treatment combinations to find the optimal temperature–humidity combination to achieve 100% phylloxera mortality over the shortest time period.
Conclusions: One hundred per cent mortality of two phylloxera clonal strains was achieved at 45°C for 75 min at 30% relative humidity. Some differences in clonal susceptibility to temperature and humidity treatments were observed.
Significance of the Study: A novel screening method was developed, which validated the nationally recognised dry heat disinfestation protocol for grape phylloxera. The study also highlighted that different clonal lineages of phylloxera are influenced by both temperature and humidity.
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