Resumen de Pleiotropic Effects of c-di-GMP Content in Pseudomonas syringae
Tingting Wang, Zhao Cai, Xiaolong Shao, Weitong Zhang, Yingpeng Xie, Yingchao Zhang, Canfeng Hua, Stephan C. Schuster, Liang Yang, Xin Deng
The present work comprehensively analyzed the transcriptome and phenotypes that were regulated by c-di-GMP in P. syringae. Given that the majority of diguanylate cyclases and phosphodiesterases have not been characterized in P. syringae, this work provided a very useful database for the future study on regulatory mechanism (especially its relationship with T3SS) of c-di-GMP in P. syringae. In particular, we identified three promoters that were sensitive to elevated c-di-GMP levels and inserted them into luciferase-based reporters that effectively respond to intracellular levels of c-di-GMP in P. syringae, which could be used as an economic and efficient way to measure relative c-di-GMP levels in vivo in the future.
