Gut Symbionts Lactobacillus reuteri R2lc and 2010 Encode a Polyketide Synthase Cluster That Activates the Mammalian Aryl Hydrocarbon Receptor

• Mustafa Özçam ^[1] ; Restituto Tocmo ^[1] ; Jee-Hwan Oh ^[1] ; Amin Afrazi ^[1] ; Joshua D. Mezrich ^[1] ; Stefan Roos ^[2] ; Jan Claesen ^[3] ; Jan-Peter van Pijkeren ^[1]
  1. [1] University of Wisconsin–Madison

     University of Wisconsin–Madison

     City of Madison, Estados Unidos

     
  2. [2] Swedish University of Agricultural Sciences

     Swedish University of Agricultural Sciences

     Uppsala domkyrkoförs., Suecia

     
  3. [3] Cleveland Clinic

     Cleveland Clinic

     City of Cleveland, Estados Unidos

     

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 10, 2019
• Idioma: inglés
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  • Temporary changes in the composition of the microbiota, for example, by oral administration of probiotics, can modulate the host immune system. However, the underlying mechanisms by which probiotics interact with the host are often unknown. Here, we show that Lactobacillus reuteri R2lc and 2010 harbor an orthologous PKS gene cluster that activates the aryl hydrocarbon receptor (AhR). AhR is a ligand-activated transcription factor that plays a key role in a variety of diseases, including amelioration of intestinal inflammation. Understanding the mechanism by which a bacterium modulates the immune system is critical for applying rational selection strategies for probiotic supplementation. Finally, heterologous and/or optimized expression of PKS is a logical next step toward the development of next-generation probiotics to prevent and treat disease.