Wheat Varietes Suitable for Celiac Patents
- Autores: María José Giménez Alvear, Javier Gil Humanes, Carmen Victoria Ozuna Serafini, Francisco Barro Losada
- Localización: Celiac Disease and Non-Celiac Gluten Sensitvity / coord. por Amado Salvador Peña; Luis Rodrigo (ed. lit.), 2014, ISBN 978-84-942118-2-9, págs. 461-476
- Idioma: inglés
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Resumen
Domestcated wheat is very complex genetcally due to its origins in an ancestral diploid species,which underwent a process of natural hybridizaton and subsequent polyploidizaton. Allcultvated wheat varietes and their wild relatves contain epitopes, which are toxic in relaton toceliac disease (CD). RNAi is an excellent tool for silencing single genes or groups of them.Combining this technology with genetc manipulaton the authors have down-regulated the toxicepitopes present in ω-, γ-, and α-gliadins of bread wheat. Monoclonal antbodies showed adecrease of almost 98% in the presence of toxic gluten. Protein extracts from those lines wereassayed using specifc T lymphocytes for DQ2 and DQ8 epitopes, showing that the new wheatlines were 100 tmes less reactve than their parental genotypes. These results represent a majorbreakthrough in achieving wheat types suitable for CD patents. The silencing of gliadins is a newbreeding trait and can be transferred by crossbreeding with elite wheat varietes. A daily intakebetween 10 and 50 mg of gluten could be safe for most CD patents, suggestng that thetransgenic lines reported here could be used in foodstufs tolerated by many CD patents.Moreover, these lines could serve as a basis for treatng other gluten pathologies such as wheat-dependent exercise-induced anaphylaxis and gluten sensitvity.
