Resumen de Study of fibroblasts activation kinetics and identification of fibroblasts subpopulations in physiological and pathological conditions
L. Carreras, A. Martínez Blanco, Christine S. Noé, J. Alcaraz Sánchez, R. Farre
Fibroblasts undergo significant morphological and functional changes in response to specific environmental cues, adopting a novel phenotype when sustaining transformative activation in wound healing and cancerous processes. The aim of this project is to characterize cytoskeletal reorganization in both control fibroblasts’ (CFs) and cancer-associated fibroblasts’ (CAFs) activation. To achieve this, we propose a novel method based on the extraction of biophysical biomarkers from epifluorescence images of the cytoskeleton of individual fibroblasts, obtained from a patient with lung cancer and activated using TGF-β. These biophysical outputs were also used to identify CAFs subpopulations. While non-tumoral fibroblasts experience larger morphological changes characterized by an increase in area and the acquisition of a robust network of actin fibers, CAFs exhibited sustained larger areas throughout the process regardless of TGF- β administration, amongst other ...
