Resumen de Measuring b-Galactosidase activity at pH 6 with a differential pH sensor

Cristina Azevedo, Matthias H Stach, Anette Amtmann, Manuel E Young, Juan G Reyes, Holger Huebner, Rainer Buchholz

• The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.