Resumen de Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
Gregorio Godoy-Hernández, Elidé Avilés-Berzunza, Mildred Carrillo-Pech, Felipe Vazquez
Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation
