Flow Injection Analysis and Liquid Chromatography for Multifunctional Chemical Analysis (MCA) Systems

• Autores: Ana V. Mayo, Thomas N. Loegel, Stacey Lowery Bretz, Neil D. Danielson
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 90, Nº 4, 2013, págs. 500-505
• Idioma: inglés
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• Resumen

  • The large class sizes of first-year chemistry labs makes it challenging to provide students with hands-on access to instrumentation because the number of students typically far exceeds the number of research-grade instruments available to collect data. Multifunctional chemical analysis (MCA) systems provide a viable alternative for large-scale instruction while supporting a hands-on approach to more advanced instrumentation. This study describes how the capabilities of MCA systems are extended to introduce liquid chromatography (LC) and flow injection analysis (FIA) in undergraduate laboratories. A semi-micro plastic cuvette with a Teflon tubing insert is fashioned as the flow cell for a MCA absorbance-fluorescence detector. Two MCA systems, Vernier and MeasureNet, are used in two unique experiments demonstrating the detection of salicylate in aspirin tablets by FIA and the LC separation of a mixture of riboflavin and fluorescein. Both instruments, composed of a syringe pump, T-injection valve, and the MCA detector, operated in the kinetic mode, are rugged and inexpensive permitting student construction, if desired.